Ribulose Diphosphate Carboxylase from Autotrophic Euglena gracilis

Autotrophic CO2 assimilation and the evolution of ribulose diphosphate carboxylase.

From Heterotrophism to Autotrophism .......... ............................... 299 New Catalysts in CO.-Fixing Autotrophs ......... .............................. 300 RuDP carboxylase and phosphoribulokinase ....... .......................... 300 Properties of RuDP Carboxylase............................................... 300 Kinetic response to substrates ........................................

Ribulose Diphosphate Carboxylase / Oxygenase

The stimulation or inhibition of ribulose diphosphate oxygenase by a variety of compounds is compared with the reported effects on these compounds on the ribulose diphosphate carboxylase activity. A possible transition state analog of ribulose diphosphate, Z-carboxyribitol 1 ,5-diphosphate, at a molar ratio of inhibitor to enzyme of 10 to 1, irreversibly inactivates the oxygenase and carboxylas...

Characterization of ribulose 1,5-bisphosphate carboxylase/oxygenase from Euglena gracilis Z.

An improved method was devised to purify ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO) with high specific activity (2.1 mumol of CO2 fixed/mg protein/min) from Euglena gracilis Z. The purified enzyme stored at -80 degrees C required treatment with dithiothreitol for full activity. The dithiothreitol-treated RuBisCO was activated by 12 mM NaHCO3 and 20 mM MgCl2, and the activated sta...

Ribulose Diphosphate Carboxylase in Thiorhodaceae.

Hydrogenase and ribulose diphosphate carboxylase during autotrophic, heterotrophic, and mixotrophic growth of scotochromogenic mycobacteria.

Two key autotrophic enzyme systems, hydrogenase and ribulose diphosphate carboxylase, were examined in Mycobacterium gordonae and two other chemolithotrophic, scotochromogenic mycobacteria under different cultural conditions. In all three organisms both enzymes were inducible and were produced in significant levels only in the presence of the specific substrate, hydrogen or carbon dioxide. M. g...